Antifouling Peptide Hydrogel Based Electrochemical Biosensors for Highly Sensitive Detection of Cancer Biomarker HER2 in Human Serum.
Wenqi WangRui HanMin ChenXi-Liang LuoPublished in: Analytical chemistry (2021)
A facile strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was presented via the fabrication of an antifouling sensing interface based on the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a biocompatible peptide hydrogel. The peptide hydrogel prepared from a designed short peptide of Phe-Glu-Lys-Phe functionalized with a fluorene methoxycarbonyl group (Fmoc-FEKF) enabled excellent activity preservation for the immobilized biomolecules, and its good hydrophilicity facilitated effective alleviation of nonspecific adsorption or biofouling, while the PEDOT film provided a highly stable and conducting substrate. The developed biosensor was highly sensitive and selective for HER2 detection, with a wide linear response range from 0.1 ng mL-1 to 1.0 μg mL-1 and a low limit of detection of 45 pg mL-1. Moreover, the peptide hydrogel based biosensor was feasible to use for complex biological samples, and it was capable of detecting HER2 in human serum with clinically acceptable accuracy, manifesting a promising potential for practical application.
Keyphrases
- label free
- sensitive detection
- loop mediated isothermal amplification
- quantum dots
- drug delivery
- epidermal growth factor receptor
- gold nanoparticles
- tissue engineering
- hyaluronic acid
- ionic liquid
- real time pcr
- wound healing
- endothelial cells
- papillary thyroid
- advanced non small cell lung cancer
- tyrosine kinase
- young adults
- high resolution
- mass spectrometry
- simultaneous determination
- climate change
- liquid chromatography
- squamous cell
- amino acid
- highly efficient