CLICK: one-step generation of conditional knockout mice.

Yoshiki MiyasakaYoshihiro UnoKazuto YoshimiYayoi KunihiroTakuji YoshimuraTomohiro TanakaHarumi IshikuboYuichi HiraokaNorihiko TakemotoTakao TanakaYoshihiro OoguchiPaul SkehelTomomi AidaJunji TakedaTomoji Mashimo

Published in: BMC genomics (2018)

The high efficiency of CLICK provides homozygous knock-ins in oocytes carrying tissue-specific Cre, which allows the one-step generation of conditional knockouts in founder (F0) mice.

Keyphrases

high efficiency
high fat diet induced
type diabetes
adipose tissue
insulin resistance
metabolic syndrome
skeletal muscle