Fabrication, microstructure characterization and degradation performance of electrospun mats based on poly(3-hydroxybutyrate-co-3 hydroxyvalerate)/polyethylene glycol blend for potential tissue engineering.
Mohamed H El-NewehyHak Yong KimTawfik A KhattabMeera Moydeen AMehrez E El-NaggarPublished in: Luminescence : the journal of biological and chemical luminescence (2021)
There have been strong demands for nanofibrous scaffolds fabricated via electrospinning for various fields due to their various advantages. Electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fiber mats were prepared. The effects of processing variables as well as the inclusion of poly (ethylene glycol) (PEG) on the morphologies of generated fibers were investigated using Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). The average fibrous diameter was monitored in the range of 400-3000 nm relying on the total content of PEG. The fluorescent cell imaging of electrospun mats was also explored. The results of cells viability demonstrated that Skin fibroblast cells BJ show different adhesions and growth rate on the three kinds of PHBV fibers. Electrospun PHBV mats with low amount of PEG offer a high-quality medium for cell growth. Thus, those mats exhibit high potential for soft tissue engineering, in particular wound healing.
Keyphrases
- tissue engineering
- induced apoptosis
- wound healing
- cell cycle arrest
- drug delivery
- high resolution
- endoplasmic reticulum stress
- cell death
- oxidative stress
- photodynamic therapy
- human health
- signaling pathway
- cell proliferation
- multiple sclerosis
- soft tissue
- risk assessment
- electron microscopy
- fluorescence imaging
- mesenchymal stem cells
- high speed