Association of mesenchymal stem cells derived from bone marrow and adipose tissue enhances bone repair in rat calvarial defects.
Gabriela Guaraldo Campos TotoliRayana Longo Bighetti-TrevisanGileade Pereira FreitasLeticia Faustino AdolphoAdriana Luisa Gonçalves AlmeidaAna Carolina Loyola BarbosaJaqueline Isadora Reis RamosAdalberto Luiz RosaAdalberto Luiz RosaPublished in: Regenerative medicine (2023)
Aim: We evaluated the bone repair induced by MSCs from adipose tissue (AT-MSCs) and bone marrow (BM-MSCs) injected into rat calvarial defects at two time points. Methods & results: Both cell populations expressed MSC surface markers and differentiated into adipocytes and osteoblasts. μCT showed that the combination of cells from distinct sources exhibited synergistic effects to increase bone repair with an advantage when BM-MSCs were injected prior to AT-MSCs. The higher osteogenic potential of these MSC combinations was demonstrated using an in vitro coculture system where BM-MSCs and AT-MSCs association induced higher ALP activity in MC3T3-E1 cells. Conclusion: Our findings may drive new approaches to treat bone defects and shed light on the complexity of the mechanisms involved in bone regeneration.
Keyphrases
- mesenchymal stem cells
- bone regeneration
- bone marrow
- umbilical cord
- adipose tissue
- cell therapy
- bone mineral density
- insulin resistance
- induced apoptosis
- high fat diet
- risk assessment
- soft tissue
- computed tomography
- drinking water
- stem cells
- single cell
- drug delivery
- endothelial cells
- image quality
- endoplasmic reticulum stress