AFEAP cloning: a precise and efficient method for large DNA sequence assembly.

Fanli ZengJinping ZangSuhua ZhangZhimin HaoJingao DongYibin Lin

Published in: BMC biotechnology (2017)

AFEAP cloning provides a powerful, efficient, seamless, and sequence-independent DNA assembly tool for multiple fragments up to 13 and large DNA up to 200 kb that expands synthetic biologist's toolbox.

Keyphrases

circulating tumor
cell free
single molecule
nucleic acid
circulating tumor cells