Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence.
Jie LiZhonghui JinYueyi GaoLei ZhouXinna GeXin GuoJun HanHanchun YangPublished in: PloS one (2017)
The recently emerged highly virulent variants of porcine epidemic and diarrhea virus (PEDV) remain a huge threat to the worldwide swine industry. Here, we describe the development of a bacterial artificial chromosome (BAC) reverse genetics system for PEDV based on two recent Chinese field isolates, namely CHM2013 and BJ2011C. Phylogenetically, CHM2013 is closely related to the vaccine strain SM98 whereas the isolate BJ2011C belongs to the GIIb group, a cluster that contains many recent pandemic strains. The full-length cDNA clones of the two isolates were constructed into BAC under the control of CMV promoter. The rescued viruses rBJ2011C and rCHM2013 were found to replicate at the kinetics similar to their respective parental viruses in cell culture. When tested in the 2-day-old pig model, rBJ2011C caused severe diarrhea of piglets with extensive damages to the intestinal epithelium, leading to 100% fatality within 48 hours. In contrast, the rCHM2013-inoculated piglets all survived with only very minor tissue damage observed. Thus, we have successfully established a convenient platform for PEDV genome manipulation. This study also represents the first description of a DNA-launched reverse genetics system for the highly virulent PEDV.
Keyphrases
- disease virus
- genetic diversity
- irritable bowel syndrome
- escherichia coli
- clostridium difficile
- copy number
- coronavirus disease
- pseudomonas aeruginosa
- dna methylation
- staphylococcus aureus
- gene expression
- magnetic resonance
- wastewater treatment
- transcription factor
- magnetic resonance imaging
- antimicrobial resistance
- biofilm formation
- computed tomography
- drug induced
- contrast enhanced