Far-red switching DNA probes for live cell nanoscopy.

Jonas BucevičiusTanja GilatGražvydas Lukinavičius

Published in: Chemical communications (Cambridge, England) (2021)

Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5'-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.

Keyphrases

single molecule
living cells
dna binding
transcription factor
fluorescent probe
atomic force microscopy
dna damage
genome wide
dna methylation
oxidative stress
solid state