Highly Stable Buffer-Based Zinc Oxide/Reduced Graphene Oxide Nanosurface Chemistry for Rapid Immunosensing of SARS-CoV-2 Antigens.
Fatemeh HaghayeghRazieh SalahandishMohsen HassaniAmir Sanati NezhadPublished in: ACS applied materials & interfaces (2022)
The widespread and long-lasting effect of the COVID-19 pandemic has called attention to the significance of technological advances in the rapid diagnosis of SARS-CoV-2 virus. This study reports the use of a highly stable buffer-based zinc oxide/reduced graphene oxide (bbZnO/rGO) nanocomposite coated on carbon screen-printed electrodes for electrochemical immuno-biosensing of SARS-CoV-2 nuelocapsid (N-) protein antigens in spiked and clinical samples. The incorporation of a salt-based (ionic) matrix for uniform dispersion of the nanomixture eliminates multistep nanomaterial synthesis on the surface of the electrode and enables a stable single-step sensor nanocoating. The immuno-biosensor provides a limit of detection of 21 fg/mL over a linear range of 1-10 000 pg/mL and exhibits a sensitivity of 32.07 ohms·mL/pg·mm 2 for detection of N-protein in spiked samples. The N-protein biosensor is successful in discriminating positive and negative clinical samples within 15 min, demonstrating its proof of concept used as a COVID-19 rapid antigen test.
Keyphrases
- reduced graphene oxide
- sars cov
- gold nanoparticles
- loop mediated isothermal amplification
- label free
- sensitive detection
- respiratory syndrome coronavirus
- protein protein
- oxide nanoparticles
- dendritic cells
- binding protein
- amino acid
- coronavirus disease
- quantum dots
- emergency department
- real time pcr
- ionic liquid
- high resolution
- immune response
- drug discovery
- electronic health record