Novel Near-Infrared Fluorescence Probe for Bioimaging and Evaluating Superoxide Anion Fluctuations in Ferroptosis-Mediated Epilepsy.
Wenjie YangRuixin LiuXiaoyi YinKe WuZhi YanXiaoming WangGuanwei FanZhixin TangYunlun LiHaiQiang JiangPublished in: Analytical chemistry (2023)
Ferroptosis is an iron-regulated, caspase-mediated pathway of cell death that is associated with the excessive aggregation of lipid-reactive oxygen species and is extensively involved in the evolution of many diseases, including epilepsy. The superoxide anion (O 2 •- ), as the primary precursor of ROS, is closely related to ferroptosis-mediated epilepsy. Therefore, it is crucial to establish a highly effective and convenient method for the real-time dynamic monitoring of O 2 •- during the ferroptosis process in epilepsy for the diagnosis and therapy of ferroptosis-mediated epilepsy. Nevertheless, no probes for detecting O 2 •- in ferroptosis-mediated epilepsy have been reported. Herein, we systematically conceptualized and developed a novel near-infrared (NIR) fluorescence probe, NIR-FP , for accurately tracking the fluctuation of O 2 •- in ferroptosis-mediated epilepsy. The probe showed exceptional sensitivity and outstanding selectivity toward O 2 •- . In addition, the probe has been utilized effectively to bioimage and evaluate endogenous O 2 •- variations in three types of ferroptosis-mediated epilepsy models (the kainic acid-induced chronic epilepsy model, the pentylenetetrazole-induced acute epilepsy model, and the pilocarpine-induced status epilepticus model). The above applications illustrated that NIR-FP could serve as a reliable and suitable tool for guiding the accurate diagnosis and therapy of ferroptosis-mediated epilepsy.
Keyphrases
- cell death
- cell cycle arrest
- living cells
- quantum dots
- reactive oxygen species
- photodynamic therapy
- temporal lobe epilepsy
- stem cells
- transcription factor
- single molecule
- nitric oxide
- oxidative stress
- high resolution
- bone marrow
- fluorescence imaging
- hydrogen peroxide
- drug delivery
- endothelial cells
- drug induced
- high glucose