Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics.
Murat SunbulJens LacknerAnnabell MartinDaniel EnglertBenjamin HaceneFranziska GrünKarin NienhausGerd Ulrich NienhausAndres JäschkePublished in: Nature biotechnology (2021)
Overcoming limitations of previous fluorescent light-up RNA aptamers for super-resolution imaging, we present RhoBAST, an aptamer that binds a fluorogenic rhodamine dye with fast association and dissociation kinetics. Its intermittent fluorescence emission enables single-molecule localization microscopy with a resolution not limited by photobleaching. We use RhoBAST to image subcellular structures of RNA in live and fixed cells with about 10-nm localization precision and a high signal-to-noise ratio.
Keyphrases
- single molecule
- living cells
- high resolution
- nucleic acid
- atomic force microscopy
- gold nanoparticles
- induced apoptosis
- fluorescent probe
- label free
- cell cycle arrest
- aqueous solution
- photodynamic therapy
- cell death
- machine learning
- endoplasmic reticulum stress
- signaling pathway
- dna binding
- high intensity
- cell proliferation
- oxidative stress