Schottiin, a new prenylated isoflavones from Psorothamnus schottii and antibacterial synergism studies between methicillin and fremontone against methicillin-resistant Staphylococcus aureus ATCC 1708.
Mallika KumarihamySiddharth K TripathiShabana KhanIlias MuhammadPublished in: Natural product research (2021)
Bioactivity guided isolation of an ethanol extract of the root of Psoromanthus schottii (Family Fabaceae) afforded a new prenylated isoflavone, named schottiin (5,7,5'-trihydroxy-4'-O-methyl-6'-(3,3-dimethylallyl)-isoflavone) (1), together with four other isoflavones, including fremontone (2), 5,7,4',5'-tetrahydroxy-2'-(3,3-dimethylallyl)-isoflavone (3), glycyrrhisoflavone (4) and fremontin (5), of which 3 and 4 identified as isomeric mixture. Structures of 1-5 were determined by full spectroscopic analyses. A comprehensive 2 D NMR spectral data has allowed revising the structure of fremontone as 2 from previously reported 2 A. Compound 2 showed weak in-vitro antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). A combination study using a checkerboard assay between fremontone (2) and methicillin exhibited a synergistic activity with 8-fold decrease in MIC of methicillin, as well as an additive effect with vancomycin against MRSA ATCC 1708. Compounds 1 and 2 also showed moderate antiplasmodial activity against chloroquine-sensitive (D6) and -resistant (W2) strains of Plasmodium falciparum with no cytotoxicity to mammalian Vero cells.
Keyphrases
- methicillin resistant staphylococcus aureus
- plasmodium falciparum
- staphylococcus aureus
- high resolution
- induced apoptosis
- escherichia coli
- magnetic resonance
- silver nanoparticles
- oxidative stress
- molecular docking
- cell cycle arrest
- high throughput
- anti inflammatory
- electronic health record
- high intensity
- mass spectrometry
- computed tomography
- cancer therapy
- cell death
- drug delivery
- cell proliferation
- case control
- endoplasmic reticulum stress
- data analysis
- dual energy