Synthesis of Poly-Ubiquitin Chains Using a Bifunctional Ubiquitin Monomer.
Gerbrand J van der Heden van NoortRaymond KooijPaul R ElliottDavid KomanderHuib OvaaPublished in: Organic letters (2017)
An optimized large scale and highly reproducible route to orthogonally protected γ-thiolysine is reported. Its utility in the synthesis of bifunctional ubiquitin monomers is demonstrated. These ubiquitin synthons are employed in polymerization reactions giving access to synthetic poly-ubiquitin chains of defined linkage.