A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells.

Charlotte R FeddersenLexy S WadsworthEliot Y ZhuHayley R VaughnAndrew P VoigtJesse D RiordanAdam J Dupuy

Published in: BMC genomics (2019)

Collectively, our work establishes a facile, adaptable method that can be performed by labs of any size to perform robust, genome-wide screens to identify genes that influence phenotypes of interest.

Keyphrases

genome wide
dna methylation
copy number
induced apoptosis
cell cycle arrest
crispr cas
endothelial cells
quantum dots
reduced graphene oxide
oxidative stress