Switchable stimulated Raman scattering microscopy with photochromic vibrational probes.
Jianpeng AoXiaofeng FangXianchong MiaoJiwei LingHyunchul KangSungnam ParkChangfeng WuMinbiao JiPublished in: Nature communications (2021)
Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding "on" or "off" SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.
Keyphrases
- single molecule
- living cells
- raman spectroscopy
- high resolution
- atomic force microscopy
- density functional theory
- label free
- molecular dynamics simulations
- visible light
- induced apoptosis
- magnetic resonance
- blood pressure
- small molecule
- high throughput
- mass spectrometry
- fluorescent probe
- cell cycle arrest
- oxidative stress
- high speed
- fluorescence imaging
- cell proliferation
- pi k akt
- molecular dynamics
- computed tomography