Comparison of Carbon Dioxide with Anaerobic Digester Biogas as a Methanogenic Biocathode Feedstock.

BES biogas upgrading studies have typically used bicarbonate or commercial gas mixtures as a biocathode substrate instead of anaerobic digester biogas. Therefore, the objective of this study was to (i) compare the performance of a methanogenic BES between CO2-fed and biogas-fed cycles; (ii) investigate possible factors that may account for observed performance differences; and (iii) assess the performance of a biogas-fed biocathode at various applied cathode potentials. The maximum 1-d CH4 production rate in a biogas-fed biocathode (3003 mmol/m2-d) was 350% higher than in a CO2-fed biocathode (666 mmol/m2-d), and the biogas-fed biocathode was capable of maintaining high performance despite a variable biogas feed composition. Anode oxidation of reduced gases (e.g., CH4 and H2S) from biogas may theoretically contribute 4% to 35% of the total charge transfer from anode to cathode at applied cathode potentials of -0.80 to -0.55 V (vs SHE). The introduction of biogas did not significantly change the biocathode archaeal community (dominated by a Methanobrevibacter sp. phylotype), but the bacterial community shifted away from Bacteroidetes and toward Proteobacteria, which may have contributed to the improved performance of the biogas-fed system. This study shows that anaerobic digester biogas is a promising biocathode feedstock for BES biogas upgrading.