Enhanced phylogenetic insights into the microbiome of chronic rhinosinusitis through the novel application of long read 16S rRNA gene amplicon sequencing.

J T ConnellK YeoG BourasA BassiouniK FenixC CooksleyS VreugdeP J WormaldA J Psaltis

Published in: Rhinology (2024)

ONT demonstrated superior resolution and culture correlations to NGS, but underestimated core sinonasal taxa. Future application and optimisation of this technology can advance our understanding of the sinonasal microenvironment.

Keyphrases

chronic rhinosinusitis
single molecule
stem cells
current status
genome wide
copy number
dna methylation
gene expression
genome wide identification