Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.
Katy PoncinAgnès RobaRavikumar JimmidiGeorges PotembergAntonella FioravantiNayla FrancisKévin WillemartNicolas ZeippenArnaud MachelartEmanuele G BiondiEric MurailleStéphane P VincentXavier De BoellePublished in: Nature communications (2019)
It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
Keyphrases
- induced apoptosis
- cell cycle
- dna repair
- transcription factor
- escherichia coli
- single cell
- cell proliferation
- stress induced
- dna damage
- genome wide
- adipose tissue
- candida albicans
- genome wide identification
- gene expression
- reactive oxygen species
- dna methylation
- crispr cas
- risk assessment
- high throughput
- multidrug resistant
- circulating tumor
- biofilm formation
- bioinformatics analysis
- gram negative
- cell cycle arrest