Cytotoxicity-Guided Isolation of Two New Phenolic Derivatives from Dryopteris fragrans (L.) Schott.
Tong ZhangLi WangDe-Hua DuanYi-Hao ZhangSheng-Xiong HuangWen Fei WangPublished in: Molecules (Basel, Switzerland) (2018)
Dryopteris fragrans is a valuable medicinal plant resource with extensive biological activities including anti-cancer, anti-oxidation, and anti-inflammation activities. This work aims to study further the cytotoxic constituents from Dryopteris fragrans. In this work, two new phenolic derivatives known as dryofragone (1) and dryofracoumarin B (2) with six known compounds (3⁻8) were isolated from the petroleum ether fraction of the methanol extract of the aerial parts of Dryopteris fragrans (L.) Schott by two round cytotoxicity-guided tracking with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and cell counting kit-8 (CCK-8) assay. Their structures were elucidated by the extensive spectroscopic analysis (¹H-NMR, 13C-NMR, and two dimensions NMR), chemical derivatization, and comparison with data reported in the literature. All the isolates were evaluated for their cytotoxicity against nine cancer cell lines as well as their in vitro immunomodulatory activity. The results showed that compounds have a modest cytotoxicity toward human HeLa cell line with IC50 value below 30 μM and compounds 4 and 5 may modulate immunity to affect the growth of tumor cells.
Keyphrases
- high resolution
- magnetic resonance
- solid state
- oxidative stress
- high throughput
- endothelial cells
- systematic review
- single cell
- molecular docking
- papillary thyroid
- electronic health record
- machine learning
- stem cells
- liquid chromatography tandem mass spectrometry
- cell therapy
- simultaneous determination
- cell death
- mass spectrometry
- artificial intelligence
- mesenchymal stem cells
- bone marrow
- induced pluripotent stem cells
- nitric oxide
- tandem mass spectrometry
- gas chromatography
- deep learning
- essential oil
- pi k akt
- clinical evaluation