tracerDB: a crowdsourced fluorescent tracer database for target engagement analysis.
Johannes DopferJames D VastaSusanne MüllerStefan KnappMatthew B RobersMartin Peter SchwalmPublished in: Nature communications (2024)
Investigating ligand-protein complexes is essential in the areas of chemical biology and drug discovery. However, detailed information on key reagents such as fluorescent tracers and associated data for the development of widely used bioluminescence resonance energy transfer (BRET) assays including NanoBRET, time-resolved Förster resonance energy transfer (TR-FRET) and fluorescence polarization (FP) assays are not easily accessible to the research community. We created tracerDB, a curated database of validated tracers. This resource provides an open access knowledge base and a unified system for tracer and assay validation. The database is freely available at https://www.tracerdb.org/ .
Keyphrases
- energy transfer
- quantum dots
- drug discovery
- high throughput
- adverse drug
- healthcare
- living cells
- positron emission tomography
- pet imaging
- electronic health record
- mental health
- social media
- emergency department
- computed tomography
- single molecule
- health information
- binding protein
- small molecule
- data analysis
- drug induced