Quantitative Elemental Analysis of a Single Cell by Using Inductively Coupled Plasma-Mass Spectrometry in Fast Time-Resolved Analysis Mode.
Yu-Ki TanakaRisako IidaShohei TakadaTetsuo KubotaMichiko YamanakaNaoki SugiyamaYolande AbdelnourYasumitsu OgraPublished in: Chembiochem : a European journal of chemical biology (2020)
The elemental composition of a single yeast, green alga, or red blood cell (RBC) was precisely determined by using inductively coupled plasma-mass spectrometry (ICP-MS) operating in fast time-resolved analysis (TRA) mode. The technique is known as single-cell (SC)-ICP-MS. Phosphorus, sulfur, magnesium, zinc, and iron were detected in the three types of cell. The elemental composition of yeast and green alga obtained by SC-ICP-MS was consistent with results obtained from conventional ICP-MS measurements following acid digestion of the cells. Slight differences were found in the measured values between SC-ICP-MS and the conventional ICP-MS results for RBC. However, the SC-ICP-MS results for S and Fe in RBC were closer to the estimated values for these elements that were calculated from the level of hemoglobin in RBCs. The data suggest that SC-ICP-MS is suitable for the analysis of various cell types, namely, fungus, plant, and animal cells.
Keyphrases
- mass spectrometry
- single cell
- red blood cell
- ms ms
- multiple sclerosis
- liquid chromatography
- high performance liquid chromatography
- capillary electrophoresis
- high resolution
- gas chromatography
- induced apoptosis
- rna seq
- cell therapy
- machine learning
- oxidative stress
- mesenchymal stem cells
- risk assessment
- data analysis
- deep learning