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Deficit of circulating CD19+ CD24hi CD38hi regulatory B cells in severe aplastic anaemia.

Yoshitaka ZaimokuBhavisha A PatelSachiko KajigayaXingmin FengLemlem AlemuDiego Quinones RaffoEmma M GroarkeNeal S Young
Published in: British journal of haematology (2020)
Immune aplastic anaemia (AA) is caused by cytotoxic T lymphocytes (CTLs) that destroy haematopoietic stem and progenitor cells. Enhanced type 1 T helper (Th1) responses and reduced regulatory T cells (Tregs) are involved in the immune pathophysiology. CD24hi CD38hi regulatory B cells (Bregs) suppress CTLs and Th1 responses, and induce Tregs via interleukin 10 (IL-10). We investigated circulating B-cell subpopulations, including CD24hi CD38hi Bregs, as well as total B cells, CD4+ T cells, CD8+ T cells and natural killer cells in 104 untreated patients with severe and very severe AA, aged ≥18 years. All patients were treated with standard immunosuppressive therapy (IST) plus eltrombopag. CD24hi CD38hi Bregs were markedly reduced in patients with AA compared to healthy individuals, especially in very severe AA, but residual Bregs remained functional, capable of producing IL-10; total B-cell counts and the other B-cell subpopulations were similar to those of healthy individuals. CD24hi CD38hi Bregs did not correlate with responses to IST, and they recovered to levels present in healthy individuals after therapy. Mature naïve B-cell counts were unexpectedly associated with IST response. Markedly reduced CD24hi CD38hi Bregs, especially in very severe AA, with recovery after IST suggest Breg deficits may contribute to the pathophysiology of immune AA.
Keyphrases
  • regulatory t cells
  • nk cells
  • early onset
  • stem cells
  • mass spectrometry
  • bone marrow
  • mesenchymal stem cells
  • acute lymphoblastic leukemia
  • immune response
  • end stage renal disease
  • cell therapy