Differences in AMY1 Gene Copy Numbers Derived from Blood, Buccal Cells and Saliva Using Quantitative and Droplet Digital PCR Methods: Flagging the Pitfall.

Delicia Shu Qin OoiVerena Ming Hui TanSiong Gim OngYiong Huak ChanChew Kiat HengYung Seng Lee

Published in: PloS one (2017)

Despite buccal cells and saliva samples being possible sources of DNA, it is pertinent that ddPCR or a single biological sample, preferably blood sample, be used for determining highly polymorphic gene copy numbers like AMY1, due to the large within-individual variability between different biological samples if real time qPCR is employed.

Keyphrases

induced apoptosis
cell cycle arrest
copy number
genome wide
endoplasmic reticulum stress
high throughput
gene expression
cell death
dna methylation
single cell
high resolution
oxidative stress
mass spectrometry
cell proliferation
cell free