Self-Assembly of Dendritic DNA into a Hydrogel: Application in Three-Dimensional Cell Culture.
Jingyuan WuBella Rosa LiyaritaHaishuang ZhuMing LiuXiao Matthew HuFangwei ShaoPublished in: ACS applied materials & interfaces (2021)
With inherent biocompatibility, biodegradability, and unique programmability, hydrogels with a DNA framework show great potential in three-dimensional (3D) cell culture. Here, a DNA hydrogel was assembled by a dendritic DNA with four branches. The hydrogel showed tunable mechanical strength and reversible thixotropy even under a nanomolar DNA concentration. The cell culture medium can be converted into the hydrogel isothermally at physiological temperature. This DNA hydrogel allows both cancer and somatic cells to be seeded in situ and to achieve high proliferation and viability. The bis-entity of dendritic branches enabled the specific loading of bioactive clues to regulate cell behaviors. Thus, the dendritic DNA-assembled hydrogel could serve as a highly biocompatible, readily functionalizing, and easy-casting gel platform for 3D cell culture.
Keyphrases
- drug delivery
- hyaluronic acid
- circulating tumor
- tissue engineering
- wound healing
- cell free
- single molecule
- nucleic acid
- squamous cell carcinoma
- signaling pathway
- single cell
- oxidative stress
- circulating tumor cells
- high throughput
- mesenchymal stem cells
- dna methylation
- gene expression
- copy number
- cell therapy
- cell proliferation
- papillary thyroid
- quantum dots
- crystal structure
- genome wide