Chemosensitizing Effect of Cernumidine Extracted from Solanum cernuum on Bladder Cancer Cells in Vitro.
Mariza A MirandaArindam MondalMandip SachdevaHamilton CabralYoussef A A H NetoIkhlas KhanMilton GroppoJames D McChesneyA N D Jairo Kenupp BastosPublished in: Chemistry & biodiversity (2019)
Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and the possible mechanism of action of the combination on bladder cancer cells. Cernumidine showed cytotoxicity and could sensitize bladder cancer cells to cisplatin. The combination of CER+cDDP inhibited cell migration on T24 cells. CER+cDDP down-regulated MMP-2/9 and p-ERK1/2, while it increased EGFR activity corroborating the observed cell migration inhibition. Down-regulation of Bcl-2 and up-regulation pro-apoptotic Bax and further depletion of the mitochondrial membrane potential (ΔΨm) indicates that mitochondria play a central role in the combination treatment inducing the mitochondrial signaling pathway of apoptosis in T24 cells. Our data showed that the alkaloid cernumidine is worthy of further studies as a chemosensitizing agent to be used in complementary chemotherapy.
Keyphrases
- cell migration
- signaling pathway
- oxidative stress
- cell death
- spinal cord injury
- pi k akt
- small cell lung cancer
- induced apoptosis
- urinary tract
- cell cycle arrest
- anti inflammatory
- endoplasmic reticulum stress
- epidermal growth factor receptor
- tyrosine kinase
- transcription factor
- big data
- risk assessment
- atomic force microscopy
- radiation therapy
- data analysis
- reactive oxygen species
- squamous cell carcinoma
- mass spectrometry
- endoplasmic reticulum
- deep learning