Conductive Thread-Based Immunosensor for Pandemic Influenza A (H1N1) Virus Detection.
Seong Uk SonSoojin JangJaewoo LimSeung Beom SeoTaejoon KangJuyeon JungSeo Yeong OhSun-Woo YoonDongeun YongJae-Jong LeeEun-Kyung LimPublished in: ACS applied materials & interfaces (2023)
Infectious agents such as viruses pose significant threats to human health, being transmitted via direct contact as well as airborne transmission without direct contact, thus requiring rapid detection to prevent the spread of infectious diseases. In this study, we developed a conductive thread-based immunosensor (CT-IS), a biosensor to easily detect the presence of airborne viruses. CT-IS utilizes an antibody that specifically recognizes the HA protein of the pandemic influenza A (pH1N1) virus, which is incorporated into the conductive thread. The antigen-antibody interaction results in increased strain on the conductive thread in the presence of the pH1N1 virus, resulting in increased electrical resistance of the CT-IS. We evaluated the performance of this sensor using the HA protein and the pH1N1 virus, in addition to samples from patients infected with the pH1N1 virus. We observed a significant change in resistance in the pH1N1-infected patient samples (positive: n = 11, negative: n = 9), whereas negligible change was observed in the control samples (patients not infected with the pH1N1 virus; negative). Hence, the CT-IS is a lightweight fiber-type sensor that can be used as a wearable biosensor by combining it with textiles, to detect the pH1N1 virus in a person's vicinity.
Keyphrases
- label free
- end stage renal disease
- computed tomography
- human health
- image quality
- sensitive detection
- sars cov
- newly diagnosed
- ejection fraction
- contrast enhanced
- risk assessment
- dual energy
- coronavirus disease
- infectious diseases
- gold nanoparticles
- loop mediated isothermal amplification
- peritoneal dialysis
- positron emission tomography
- particulate matter
- climate change
- prognostic factors
- magnetic resonance
- tissue engineering
- air pollution