Label-free nanoscale optical metrology on myelinated axons in vivo.
Junhwan KwonMoonseok KimHyejin ParkBok-Man KangYongjae JoJae-Hwan KimOliver JamesSeok-Hyun YunSeong-Gi KimMinah SuhMyunghwan ChoiPublished in: Nature communications (2017)
In the mammalian nervous system, myelin provides electrical insulation for the neural circuit by forming a highly organized, multilayered thin film around the axon fibers. Here, we investigate the spectral reflectance from this subcellular nanostructure and devise a new label-free technique based on a spectroscopic analysis of reflected light, enabling nanoscale imaging of myelinated axons in their natural living state. Using this technique, we demonstrate three-dimensional mapping of the axon diameter and sensing of dynamic changes in the substructure of myelin at nanoscale. We further reveal the prevalence of axon bulging in the brain cortex in vivo after mild compressive trauma. Our novel tool opens new avenues of investigation by creating unprecedented access to the nanostructural dynamics of live myelinated axons in health and disease.
Keyphrases
- label free
- optic nerve
- high resolution
- atomic force microscopy
- white matter
- high speed
- optical coherence tomography
- healthcare
- public health
- resting state
- functional connectivity
- molecular docking
- risk factors
- mental health
- single cell
- gene expression
- health information
- magnetic resonance
- magnetic resonance imaging
- dna methylation
- risk assessment
- health promotion
- single molecule
- trauma patients
- contrast enhanced