wFLFM: enhancing the resolution of Fourier light-field microscopy using a hybrid wide-field image.

Published in: Applied physics express (2021)

We introduce wFLFM, an approach that enhances the resolution of Fourier light-field microscopy (FLFM) through a hybrid wide-field image. The system exploits the intrinsic compatibility of image formation between the on-axis FLFM elemental image and the wide-field image, allowing for minimal instrumental and computational complexity. The numerical and experimental results of wFLFM present a two- to three-fold improvement in the lateral resolution without compromising the 3D imaging capability in comparison with conventional FLFM.

Keyphrases

deep learning
single molecule
high resolution
high throughput
machine learning
photodynamic therapy