Fractionation of bacteria by electrophoresis as pre-separation method before MALDI-MS detection.
Dominika BłońskaEwa Maria KłodzińskaBoguslaw BuszewskiPublished in: Electrophoresis (2023)
The present study attempted to apply the capillary electrophoresis technique for the fractionation and separation of S. Staphylococcus hominis and Escherichia coli bacteria isolated from urine samples and the detection of migrated fraction with spectrometric method. This involved the selection of suitable conditions for separation as well as the identification of pathogens. The result of the research was the separation of Gram-negative and Gram-positive bacteria, as well as their subsequent identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using two different approaches (culture of fractions on an agar plate and direct analysis of the collected fractions). The preliminary results provide a solid basis for further research on the use of electromigration techniques with LDI detection to identify pathogens such as bacteria and viruses in biological samples.
Keyphrases
- gram negative
- liquid chromatography
- mass spectrometry
- multidrug resistant
- capillary electrophoresis
- escherichia coli
- loop mediated isothermal amplification
- real time pcr
- multiple sclerosis
- tandem mass spectrometry
- staphylococcus aureus
- high performance liquid chromatography
- klebsiella pneumoniae
- biofilm formation
- bioinformatics analysis
- antimicrobial resistance