Liquid application method for time-resolved analyses by serial synchrotron crystallography.
Pedram MehrabiEike-Christian SchulzMichael AgtheSam HorrellGleb BourenkovDavid von StettenJan-Philipp LeimkohlHendrik SchikoraThomas R SchneiderArwen R PearsonFriedjof TellkampR J Dwyane MillerPublished in: Nature methods (2019)
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.