Long-term imaging of intranuclear Mg 2+ dynamics during mitosis using a localized fluorescent probe.

A novel fluorescent Mg 2+ probe was developed based on a small molecule-protein hybrid. This probe enables subcellular targeting, long-term imaging, and high selectivity for Mg 2+ over Ca 2+ . Using ratiometric fluorescence microscopy with a co-localized standard fluorophore, the variations in intranuclear Mg 2+ concentrations during mitosis could be visualized.