QuEChERS LC-MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices.
Licia PantanoLadislao La ScalaFrancesco OlibrioFrancesco Giuseppe GalluzzoCarmelo BongiornoMaria Drussilla BuscemiAndrea MacalusoAntonio VellaPublished in: International journal of environmental research and public health (2021)
We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB1, AFB2, AFG1, AFG2), deoxynivalenol (DON), fumonisin (FUMO; FB1, FB2, FB3), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method's performance was assessed by two different proficiencies and tested with 100 real samples.
Keyphrases
- tandem mass spectrometry
- ultra high performance liquid chromatography
- ms ms
- gas chromatography
- simultaneous determination
- high performance liquid chromatography
- solid phase extraction
- liquid chromatography
- high resolution mass spectrometry
- liquid chromatography tandem mass spectrometry
- high resolution
- mass spectrometry
- gas chromatography mass spectrometry
- ionic liquid
- loop mediated isothermal amplification