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Pig blastocyst-like structure models from embryonic stem cells.

Jinzhu XiangHanning WangBingbo ShiJiajun LiDong LiuKaipeng WangZhuangfei WangQiankun MinChengchen ZhaoDuan-Qing Pei
Published in: Cell discovery (2024)
Pluripotent stem cells have the potential to generate embryo models that can recapitulate developmental processes in vitro. Large animals such as pigs may also benefit from stem-cell-based embryo models for improving breeding. Here, we report the generation of blastoids from porcine embryonic stem cells (pESCs). We first develop a culture medium 4FIXY to derive pESCs. We develop a 3D two-step differentiation strategy to generate porcine blastoids from the pESCs. The resulting blastoids exhibit similar morphology, size, cell lineage composition, and single-cell transcriptome characteristics to blastocysts. These porcine blastoids survive and expand for more than two weeks in vitro under two different culture conditions. Large animal blastoids such as those derived from pESCs may enable in vitro modeling of early embryogenesis and improve livestock species' breeding practices.
Keyphrases
  • embryonic stem cells
  • single cell
  • rna seq
  • stem cells
  • healthcare
  • primary care
  • gene expression
  • dna methylation
  • gestational age
  • transcription factor
  • bone marrow
  • preterm birth