Characterization and Engineering of the Type 3 Secretion System Needle Monomer from Salmonella Through the Construction and Screening of a Comprehensive Mutagenesis Library.

Lisa Ann BurdetteSamuel Alexander LeachNolan W KennedyBon C IkwuagwuJordan S SummersDanielle Tullman-Ercek

Published in: bioRxiv : the preprint server for biology (2024)

can impact secretion titer. We demonstrate that this complex is amenable to comprehensive mutagenesis studies and that this can yield both PrgI variants with increased secretory capabilities and insight into the normal functioning of the type 3 secretion system.

Keyphrases

crispr cas
escherichia coli
ultrasound guided
copy number
molecularly imprinted
mass spectrometry
genome wide
simultaneous determination
solid phase extraction