Bacterial Extracellular DNA Production Is Associated with Outcome of Prosthetic Joint Infections.
Beata ZatorskaCarla Renata ArciolaNicolas HaffnerLuigi Segagni LusignaniElisabeth PresterlMagda Diab-ElschahawiPublished in: BioMed research international (2018)
In a retrospective study the association of the production of extracellular DNA (eDNA) in biofilms of clinical staphylococcal isolates from 60 patients with prosthetic joint infection (PJI) and the clinical outcome were investigated. Data from a previous study on eDNA production determined in 24-hour biofilms of staphylococcal isolates (Staphylococcus aureus n=30, Staphylococcus epidermidis n=30) was correlated with the patients' clinical outcome after 3 and 12 months. Statistical analysis was performed using either the Spearman's rank correlations test or the t-test. eDNA production of S. epidermidis in 24-hour biofilms correlated with the patients' outcome 'not cured' after 12 months. For S. aureus no such correlation was detected. Thus, eDNA may be a virulence factor of S. epidermidis. Quantification of eDNA production as a surrogate marker for biofilm formation might be a potential predictive marker for the management of PJI.
Keyphrases
- biofilm formation
- staphylococcus aureus
- candida albicans
- pseudomonas aeruginosa
- end stage renal disease
- escherichia coli
- ejection fraction
- newly diagnosed
- chronic kidney disease
- peritoneal dialysis
- methicillin resistant staphylococcus aureus
- cystic fibrosis
- patient reported outcomes
- single molecule
- cell free
- high resolution
- human health