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Identification and characterization of two novel ISCR1-associated genes dfrA42 and dfrA43 encoding trimethoprim resistant dihydrofolate reductases.

Ling LiMengge ZhangWenjia WangRuirui XiaYanan MaXin WeiXinhua WangXiaomin SunXiaohong XieShiling XieMingyu WangHai Xu
Published in: Antimicrobial agents and chemotherapy (2021)
Two novel ISCR1-associated dfr genes, dfrA42 and dfrA43, were identified from trimethoprim (TMP)-resistant Proteus strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into Escherichia coli These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.
Keyphrases
  • escherichia coli
  • genome wide
  • bioinformatics analysis
  • genome wide identification
  • dna methylation
  • mass spectrometry
  • pseudomonas aeruginosa
  • cystic fibrosis
  • nitric oxide
  • multidrug resistant
  • candida albicans