Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris).

Mihail BesleagaGabriel A VignolleJulian KoppOliver SpadiutRobert L MachAstrid R Mach-AignerChristian Zimmermann

Published in: Fungal biology and biotechnology (2023)

The usage of ACT1 as a reference gene in RT-qPCR analysis might lead to distorted results due to the instability of its transcript levels. In this study, we evaluated the transcript levels of several genes and found RSC1 and TAF10 to be extremely stable. Using these genes holds the promise for reliable RT-qPCR results.

Keyphrases

genome wide
genome wide identification
rna seq
bioinformatics analysis
genome wide analysis
dna methylation
copy number
transcription factor
recombinant human
single cell
gene expression
big data
deep learning
artificial intelligence
data analysis