Facile manufacturing of fused deposition modelled composite scaffold for tissue engineering - An embedment model with plasticity for incorporation of additives.

Fused deposition modeling (FDM) process is carried out at an elevated temperature, preventing the addition of biological factors, drugs, bioactive compounds, etc., during fabrication. To surpass this disadvantage, 3D interlinked porous PLA (Polylactic acid) scaffold was fabricated by FDM, followed by embedment of PCL (polycaprolactone) scaffold into the pores of PLA at room temperature yielding PLA-PCL scaffold. In addition, PLA-PCL scaffold with nanohydroxyapatite (PLA-PCL-nHAP) and multiwalled carbon nanotubes (PLA-PCL-MWCNT) were also fabricated. Herein, FDM fabricated PLA scaffold functions as a "structural component" whereas embedded PCL scaffold acts as "functional component" which provides a provision to functionalize the scaffolds with desired chemical or biological materials. The embedment process is straightforward, cost effective, and does not require sophistication. Mechanical characterization of scaffolds suggests Young's modulus of PLA-PCL scaffold (16.02 MPa) was higher than FDM fabricated PLA (9.98 MPa) scaffold by virtue of embedded PCL matrix. Besides, Finite element analysis showed, von Mises stress on mandible with scaffolds at 4.04 MPa, whereas mandible with the defect was 6.7 MPa suggesting stress distribution efficiency and mechanical stability of these scaffolds. Further, field emission scanning electron microscope (FESEM) analysis implied interlinked porous structures with a pore diameter of 50 µm to 300 µm. X-Ray diffraction (XRD) results revealed an increased crystallinity (%) of embedment models (PLA-PCL, PLA-PCL-nHAP and PLA-PCL-MWCNT) compared to PLA printed scaffold. Additionally, Raman analysis revealed that the embedment process did not impart chemical alterations in polymeric chains. In vitro analysis with human osteoblasts exhibited osteoconductive nature of the scaffold by supporting mineralization. In brief, the advantages the model is that, it helps to overcome the hassles of manufacturing a filament with desired additives for FDM, and offers provision to incorporate desired concentrations of heat labile bioactive molecules during embedment process at ambient temperature.