Cancer Cell-Specific and Laser-Activatable NanoSeeds for Targeted Photothermal Ablation of Triple-negative Breast Cancer.
Dnyaneshwar KalyaneSuryanarayana PolakaNupur VasdevRakesh Kumar TekadePublished in: Photochemistry and photobiology (2022)
This investigation reports the quality-by-design (QbD) assisted novel templated approach for developing cancer cell-specific and laser-activatable nanoseeds (AuraTherm) for targeted photothermal ablation of triple negative breast cancer (TNBC). AuraTherm was nanometric in size as characterized by SEM, TEM, and particle analysis (75.15 ± 3.11 nm; -21.80 ±0.17 mV) with hemocompatibility and neutrality towards blood components. AuraTherm showed reversible photothermal effect (ΔT: 37±1.2 °C → 49.4±1.5 °C; 15 min; 2.4 W/cm 2 ) employing near infrared-808 nm laser (NIR-808). The targeted cytosolic localization led to a significant anticancer activity as evaluated using apoptosis assay, cell cycle analysis, Intracellular ROS generation assay, cellular uptake, and receptor binding assay. The NIR-808 laser-responsive photothermal ablation of cancer cell was found to be more effective compared to without NIR-808 laser-treated counterparts, suggesting the fundamental role of photothermal ablation in the treatment of TNBC.
Keyphrases
- photodynamic therapy
- cancer therapy
- drug release
- fluorescence imaging
- cell cycle
- drug delivery
- high throughput
- fluorescent probe
- radiofrequency ablation
- high speed
- cell death
- oxidative stress
- emergency department
- catheter ablation
- reactive oxygen species
- dna damage
- quality improvement
- high resolution
- newly diagnosed
- smoking cessation
- light emitting