A wide-band bio-chip for real-time optical detection of bioelectromagnetic interactions with cells.
Caterina MerlaMicaela LibertiPaolo MarracinoAdeline MuscatAntoine AzanFrancesca ApollonioLluis M MirPublished in: Scientific reports (2018)
The analytical and numerical design, implementation, and experimental validation of a new grounded closed coplanar waveguide for wide-band electromagnetic exposures of cells and their optical detection in real-time is reported. The realized device fulfills high-quality requirements for novel bioelectromagnetic experiments, involving elevated temporal and spatial resolutions. Excellent performances in terms of matching bandwidth (less than -10 dB up to at least 3 GHz), emission (below 1 × 10-6 W/m2) and efficiency (around 1) have been obtained as revealed by both numerical simulations and experimental measurements. A low spatial electric field inhomogeneity (coefficient of variation of around 10 %) has been achieved within the cell solutions filling the polydimethylsiloxane reservoir of the conceived device. This original bio-chip based on the grounded closed coplanar waveguide concept opens new possibilities for the development of controlled experiments combining electromagnetic exposures and sophisticated imaging using optical spectroscopic techniques.
Keyphrases
- high resolution
- induced apoptosis
- cell cycle arrest
- high speed
- air pollution
- high throughput
- healthcare
- high frequency
- endoplasmic reticulum stress
- loop mediated isothermal amplification
- primary care
- cell death
- single cell
- molecular docking
- molecular dynamics
- magnetic resonance
- cell therapy
- cell proliferation
- magnetic resonance imaging
- quality improvement
- computed tomography
- bone marrow
- liquid chromatography
- diffusion weighted imaging
- mesenchymal stem cells
- contrast enhanced
- monte carlo