Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods.
Bethan Lloyd-LewisFelicity Mae DavisOlivia B HarrisJessica R HitchcockFilipe C LourencoMathias PascheChristine J WatsonPublished in: Breast cancer research : BCR (2016)
The utility of whole-organ tissue clearing protocols was assessed in the mouse mammary gland. Most methods utilised affordable and widely available reagents, and were compatible with standard confocal microscopy. These techniques enable high-resolution, 3D imaging and phenotyping of mammary cells and tumours in situ, and will significantly enhance our understanding of both normal and pathological mammary gland development.