Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding.
Mark C LeakePublished in: Transcription (2018)
High-speed single-molecule fluorescence microscopy in vivo shows that transcription factors in eukaryotes can act in oligomeric clusters mediated by molecular crowding and intrinsically disordered protein. This finding impacts on the longstanding puzzle of how transcription factors find their gene targets so efficiently in the complex, heterogeneous environment of the cell. Abbreviations CDF - cumulative distribution function; FRAP - fluorescence recovery after photobleaching; GFP - Green fluorescent protein; STORM - stochastic optical reconstruction microscopy; TF - Transcription factor; YFP - Yellow fluorescent protein.
Keyphrases
- single molecule
- transcription factor
- high speed
- atomic force microscopy
- living cells
- gene expression
- protein protein
- high resolution
- dna binding
- amino acid
- binding protein
- quantum dots
- dna methylation
- induced apoptosis
- single cell
- small molecule
- high throughput
- cell therapy
- optical coherence tomography
- stem cells
- oxidative stress
- signaling pathway
- bone marrow
- cell death