Detection of several carbapenems resistant and virulence genes in classical and hyper-virulent strains of Klebsiella pneumoniae isolated from hospitalized neonates and adults in Khartoum.
Aalaa Mahgoub AlbashaEsraa Hassan OsmanSaga Abd-AlhalimElianz F AlshaibLeena Al-HassanHisham N AltaybPublished in: BMC research notes (2020)
Seventy percent of the isolates were resistant to ceftazidime, 18(30%) to ciprofloxacin, 23(38.3%) to chloramphenicol, 24(40%) to gentamicin and 8% to imipenem, 35% were multidrug-resistant, and 7% extensively drug-resistant, all blood isolates (n = 14) were resistant to ceftazidime. entB was the most predominant virulence gene (93.3%), followed by mrkD (78.3%), kfu (60%), K2 (51.7%), magA (18.3%) and rmpA (5%). blaOXA-48 was the most predominant carbapenem-resistant gene (68.3%), followed by blaNDM (10%), blaKPC (8.3%), and blaIMP (3.3%). Eight hyper-virulent strains were positive for blaOXA-48 and two for blaNDM genes.
Keyphrases
- klebsiella pneumoniae
- multidrug resistant
- drug resistant
- gram negative
- escherichia coli
- acinetobacter baumannii
- genome wide identification
- genome wide
- pseudomonas aeruginosa
- staphylococcus aureus
- copy number
- genome wide analysis
- biofilm formation
- antimicrobial resistance
- genetic diversity
- transcription factor
- bioinformatics analysis
- gene expression
- low birth weight
- loop mediated isothermal amplification
- sensitive detection