Exploring capabilities of elemental mass spectrometry for determination of metal and biomolecules in extracellular vesicles.
Jaime Martínez-GarcíaAlicia Villa-VázquezBeatriz FernándezHéctor González-IglesiasRosario PereiroPublished in: Analytical and bioanalytical chemistry (2023)
Extracellular vesicles (EVs) are increasingly recognized as crucial components influencing various pathophysiological processes, such as cellular homeostasis, cancer progression, and neurological disease. However, the lack of standardized methods for EV isolation and classification, coupled with ambiguity in biochemical markers associated with EV subtypes, remains a major challenge. This Trends article highlights the most common approaches for EV isolation and characterization, along with recent applications of elemental mass spectrometry (MS) to analyse metals and biomolecules in EVs obtained from biofluids or in vitro cellular models. Considering the promising capabilities of elemental MS, the article also looks ahead to the potential analysis of EVs at the single-vesicle and single-cell levels using ICP-MS. These approaches may offer valuable insights into individual characteristics of EVs and their functions, contributing to a deeper understanding of their role in various biological processes.
Keyphrases
- mass spectrometry
- liquid chromatography
- single cell
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- high resolution
- multiple sclerosis
- machine learning
- papillary thyroid
- human health
- ms ms
- solid phase extraction
- deep learning
- tandem mass spectrometry
- risk assessment
- squamous cell
- blood brain barrier
- drinking water
- molecularly imprinted
- heavy metals
- lymph node metastasis