Dual Functional Ultrasensitive Point-of-Care Clinical Diagnosis Using Metal-Organic Frameworks-Based Immunobeads.
Fengli ChaiDou WangFei ShiWenfu ZhengXiaohui ZhaoYao ChenCuiping MaoJiangjiang ZhangXingyu JiangPublished in: Nano letters (2023)
Sepsis is an acute systemic infectious syndrome with high fatality. Fast and accurate diagnosis, monitoring, and medication of sepsis are essential. We exploited the fluorescent metal-AIEgen frameworks (MAFs) and demonstrated the dual functions of protein detection and bacteria identification: (i) ultrasensitive point-of-care (POC) detection of sepsis biomarkers (100 times enhanced sensitivity); (ii) rapid POC identification of Gram-negative/positive bacteria (selective aggregation within 20 min). Fluorescent lateral flow immunoassays (LFAs) are convenient and inexpensive for POC tests. MAFs possess a large surface area, excellent photostability, high quantum yield (∼80%), and multiple active sites serving as protein binding domains for ultrasensitive detection of sepsis biomarkers (IL-6/PCT) on LFAs. The limit of detection (LOD) for IL-6/PCT is 0.252/0.333 pg/mL. Rapid appraisal of infectious bacteria is vital to guide the use of medicines. The dual-functional fluorescent MAFs have great potential in POC tests for the clinical diagnosis of bacterial infections.
Keyphrases
- label free
- loop mediated isothermal amplification
- quantum dots
- acute kidney injury
- intensive care unit
- septic shock
- gram negative
- sensitive detection
- gold nanoparticles
- multidrug resistant
- real time pcr
- metal organic framework
- healthcare
- liver failure
- emergency department
- binding protein
- amino acid
- small molecule
- risk assessment
- protein protein
- mass spectrometry
- molecularly imprinted
- human health
- climate change
- transcription factor
- adverse drug
- bioinformatics analysis