Non-thermal plasma elicits ferrous chloride-catalyzed DMPO-OH.
Yasumasa OkazakiNanami ItoHiromasa TanakaMasaru HoriShinya ToyokuniPublished in: Free radical research (2022)
Non-thermal plasma (NTP) induces the generation of reactive oxygen species (ROS) and reactive nitrogen species, such as hydroxyl radicals ( • OH), hydrogen peroxide (H 2 O 2 ), singlet oxygen, superoxide, ozone, and nitric oxide, at near-physiological temperatures. These molecules promote blood coagulation, wound healing, disinfection, and selective cancer cell death. Based on these evidences, clinical trials of NTP have been conducted for treating chronic wounds and head and neck cancers. Although clinical applications have progressed, the stoichiometric quantification of NTP-induced ROS remains unclear in the liquid phase in the presence of FeCl 2 or FeCl 3 in combination with biocompatible reducing agents, which may modulate the final biological effects of NTP. In this study, we employed electron paramagnetic resonance spectroscopy to quantify ROS using spin-trapping probe, 5,5-dimethyl-1-pyrroline- N -oxide (DMPO) and H 2 O 2 , using luminescent probe in the presence of FeCl 2 or FeCl 3 . NTP-induced DMPO-OH levels were elevated 10-100 µM FeCl 2 or 500 and 1000 µM FeCl 3 . NTP-induced DMPO-OH with 10 µM FeCl 2 or FeCl 3 was significantly scavenged by ascorbate, α-tocopherol, dithiothreitol, reduced glutathione, or oxidized glutathione, whereas dehydroascorbate was ineffective in 2 mM DMPO. NTP-induced H 2 O 2 was significantly degraded by 100 µM FeCl 2 and FeCl 3 in an iron-dependent manner. Meanwhile, decomposition of H 2 O 2 by catalase decayed DMPO-OH efficiently in the presence of iron, indicating iron causes DMPO-OH production and degradation simultaneously. These results suggest that NTP-induced DMPO-OH is generated by the H 2 O 2 -consuming, iron-dependent Fenton reaction and ferryl intermediates. The potential iron-mediated ROS production by NTP is also discussed to clarify the interaction between NTP-induced ROS and biomolecules.
Keyphrases
- cell death
- hydrogen peroxide
- reactive oxygen species
- high glucose
- nitric oxide
- diabetic rats
- clinical trial
- dna damage
- drug induced
- squamous cell carcinoma
- endothelial cells
- oxidative stress
- room temperature
- mass spectrometry
- risk assessment
- signaling pathway
- air pollution
- cell cycle arrest
- ionic liquid
- lymph node metastasis
- genetic diversity
- energy transfer
- nitric oxide synthase
- human health
- stress induced
- electron microscopy