Time dependent impact of perinatal hypoxia on growth hormone, insulin-like growth factor 1 and insulin-like growth factor binding protein-3.
Ömer KartalSeçil AydınözAyşe Tuğba KartalTaha KelestemurAhmet Burak CaglayanMustafa Caglar BekerFerhan KarademirSelami SüleymanoğluMustafa KulBurak YulugErtugrul KilicPublished in: Metabolic brain disease (2016)
Hypoxic-ischemia (HI) is a widely used animal model to mimic the preterm or perinatal sublethal hypoxia, including hypoxic-ischemic encephalopathy. It causes diffuse neurodegeneration in the brain and results in mental retardation, hyperactivity, cerebral palsy, epilepsy and neuroendocrine disturbances. Herein, we examined acute and subacute correlations between neuronal degeneration and serum growth factor changes, including growth hormone (GH), insulin-like growth factor 1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) after hypoxic-ischemia (HI) in neonatal rats. In the acute phase of hypoxia, brain volume was increased significantly as compared with control animals, which was associated with reduced GH and IGF-1 secretions. Reduced neuronal survival and increased DNA fragmentation were also noticed in these animals. However, in the subacute phase of hypoxia, neuronal survival and brain volume were significantly decreased, accompanied by increased apoptotic cell death in the hippocampus and cortex. Serum GH, IGF-1, and IGFBP-3 levels were significantly reduced in the subacute phase of HI. Significant retardation in the brain and body development were noted in the subacute phase of hypoxia. Here, we provide evidence that serum levels of growth-hormone and factors were decreased in the acute and subacute phase of hypoxia, which was associated with increased DNA fragmentation and decreased neuronal survival.
Keyphrases
- growth hormone
- cerebral ischemia
- cell death
- binding protein
- growth factor
- resting state
- endothelial cells
- white matter
- functional connectivity
- cerebral palsy
- liver failure
- subarachnoid hemorrhage
- pregnant women
- brain injury
- blood brain barrier
- mental health
- circulating tumor
- single molecule
- free survival
- intensive care unit
- early onset
- cell free
- multiple sclerosis
- aortic dissection