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Freezing the graft is not necessary for autotransplants for plasma cell myeloma and lymphomas.

Amado J Karduss-UruetaRobert Peter GaleCésar H Gutierrez-AguirreMiguel Angel Herrera-RojasIván Murrieta-ÁlvarezRosendo Perez-FontalvoGuillermo J Ruiz-DelgadoGiovanni Ruiz-RojasGregorio JaimovichLeonardo FeldmanNancy Labastida-MercadoAlicia EndaraMartín CastroSamantha Galindo-BecerraMaría Angélica Cardona-MolinaDavid Gomez-AlmaguerGuillermo José Ruiz-Arguelles
Published in: Bone marrow transplantation (2018)
We studied rates of granulocyte and platelets recovery in 359 consecutive subjects receiving blood cell infusions in the context of autotransplants for plasma cell myeloma (N = 216) and lymphomas (N = 143). Blood cells were mobilised with filgrastim given for 4-5 days and collected after a median of 2 (range, 1-2) apheresis. Apheresis products were stored at 4° C for a median of 3 days (range, 2-6 days). Most subjects received carmustine, etoposide, cytarabine and melphalan (BEAM), cyclophosphamide, carmustine and etoposide (CBV) or high-dose melphalan. Filgrastim was given post transplant to 319 subjects. Median numbers of mononuclear cells collected was 31 × 10E + 6/kg (interquartile range (IQR) 37 × 10E + 6 cells/kg). Median numbers of CD34-positive cells collected was 3.6 × 10E + 6/kg (IQR 3.8 × 10E + 6/Kg). Median viability after collection was 90% (IQR 7%) after storage, 88% (IQR 12%). A total of 255 of 256 evaluable subjects recovered bone marrow function and there was no late bone marrow failure. Median interval to neutrophils >0.5 × E + 9/L was 13 days (range, 9-39 days) and to platelets >20 × 10E + 9/L, 16 days (range, 7-83 days). These rates and ranges seem comparable to those reported after autotransplants of frozen blood cells. There was no correlation between numbers of storage days at 4 °C and viability afte storage (r = -0.018, p = 0.14)) nor rates of recovery of neutrophils (r = -0.054, p = 0.52) or platelets (r = 0.116, p = 0.14). Blood cells collected for autotransplant can be stored at 4 °C for 6 d. This method is simple, inexpensive and widely applicable.
Keyphrases
  • induced apoptosis
  • high dose
  • cell cycle arrest
  • bone marrow
  • low dose
  • single cell
  • mesenchymal stem cells
  • endoplasmic reticulum stress
  • stem cells
  • cell therapy
  • signaling pathway
  • peripheral blood
  • acute myeloid leukemia