Antiquorum sensing and antibiofilm potential of biosynthesized silver nanoparticles of Myristica fragrans seed extract against MDR Salmonella enterica serovar Typhi isolates from asymptomatic typhoid carriers and typhoid patients.
Senthilkumar BalakrishnanKalibulla Syed IbrahimSenbagam DuraisamyIlakkia SivajiSelvam KandasamyAnbarasu KumarasamyNachimuthu Senthil KumarPublished in: Environmental science and pollution research international (2019)
Globally, Salmonella infection poses a major public health problem. Here, we report antibiofilm activity and quorum sensing inhibition of aqueous seeds extract of Myristica fragrans (nutmeg) and biosynthesized silver nanoparticles (AgNPs) against multidrug resistant (MDR) Salmonella enterica serovar Typhi (S. Typhi) isolated from typhoid patients and asymptomatic carriers. S. Typhi isolates revealed higher percentage (46%) of biofilm production identified by tissue culture plate (TCP) than Congo red agar (CRA) and tube adherence (TA) methods. The inhibition of biofilm-producing MDR S. Typhi isolates and pigment production of Chromobacterium violaceum (indicator bacteria) demonstrated the quorum sensing potential of nutmeg. The aqueous seed extract of nutmeg exhibited 87% of antibiofilm activity, while the biosynthesized AgNPs showed 99.1% of antibiofilm activity. Molecular docking studies of bioactive compounds of nutmeg against transcriptional regulatory protein RcsB and sensor kinase protein RcsC revealed interaction with the target proteins. It is proposed that biosynthesized AgNPs could be used as one of the effective candidates in treating asymptomatic typhoid carriers or typhoid patients and to control the subsistence of biofilm-producing S. Typhi strains or other pathogenic bacteria in the environment or industrial settings.
Keyphrases
- silver nanoparticles
- multidrug resistant
- end stage renal disease
- public health
- ejection fraction
- newly diagnosed
- molecular docking
- pseudomonas aeruginosa
- chronic kidney disease
- escherichia coli
- oxidative stress
- prognostic factors
- gene expression
- patient reported outcomes
- biofilm formation
- transcription factor
- drug resistant
- molecular dynamics simulations
- cystic fibrosis
- small molecule
- climate change
- adipose tissue
- amino acid
- patient reported
- skeletal muscle
- heat shock protein
- ionic liquid
- klebsiella pneumoniae