Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells.
Mun-Ki ChoiGil-Sung KimJin-Tak JeongJung-Taek LimWon-Yong LeeAhmad UmarSang-Kwon LeePublished in: Scientific reports (2017)
The detection of cancer biomarkers has recently attracted significant attention as a means of determining the correct course of treatment with targeted therapeutics. However, because the concentration of these biomarkers in blood is usually relatively low, highly sensitive biosensors for fluorescence imaging and precise detection are needed. In this study, we have successfully developed vertical GaN micropillar (MP) based biosensors for fluorescence sensing and quantitative measurement of CA15-3 antigens. The highly ordered vertical GaN MP arrays result in the successful immobilization of CA15-3 antigens on each feature of the arrays, thereby allowing the detection of an individual fluorescence signal from the top surface of the arrays owing to the high regularity of fluorophore-tagged MP spots and relatively low background signal. Therefore, our fluorescence-labeled and CA15-3 functionalized vertical GaN-MP-based biosensor is suitable for the selective quantitative analysis of secreted CA15-3 antigens from MCF-7 cell lines, and helps in the early diagnosis and prognosis of serious diseases as well as the monitoring of the therapeutic response of breast cancer patients.
Keyphrases
- label free
- loop mediated isothermal amplification
- fluorescence imaging
- real time pcr
- single molecule
- high density
- high resolution
- squamous cell carcinoma
- gold nanoparticles
- energy transfer
- machine learning
- deep learning
- cancer therapy
- light emitting
- sensitive detection
- papillary thyroid
- mass spectrometry
- immune response
- young adults
- breast cancer cells
- pet ct
- squamous cell
- simultaneous determination