Determination of the Chromatin Openness in Bacterial Genomes.
Mahmoud M Al-BassamKarsten ZenglerPublished in: Methods in molecular biology (Clifton, N.J.) (2023)
The hyperactive Tn5 transposase in the ATAC-seq method has been widely used to determine the open DNA regions and understand the overall epigenomic regulation in the chromatins of eukaryotic cells. Here, we describe POP-seq (Prokaryotic chromatin Openness Profiling sequencing), an adaptation of the ATAC-seq method, to interrogate changes in the openness of prokaryotic nucleoids.
Keyphrases
- single cell
- genome wide
- rna seq
- dna methylation
- induced apoptosis
- gene expression
- dna damage
- transcription factor
- cell cycle arrest
- minimally invasive
- single molecule
- circulating tumor
- cell free
- endoplasmic reticulum stress
- oxidative stress
- molecularly imprinted
- high resolution
- cell proliferation
- nucleic acid
- pi k akt